Application

0·3% Triton^™ X-100 in TBS has been used for permeabilization of retinas before immunostaining. 0.5% Triton X-100 has been used for astrocyte cell lysis.

Biochem/physiol Actions

Widely used non-ionic surfactant for recovery of membrane components under mild non-denaturing conditions.

General description

Triton^™ X-100 is a common non-ionic surfactant and emulsifier which is often used in biochemical applications to solubilize proteins. It is considered a comparatively mild detergent, non-denaturing, and is reported in numerous references as a routinely added reagent. It is utilized for lysing cells to extract protein and cellular organelles. It can also permeabilize the living cell membrane for transfection.

Legal Information

Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow

Packaging

5, 5×5, 10×5 mL in ampule

Packaged in ampules under Argon.